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Code Name Applications Availability Size Tested species Price Grade  
EB09857-B Goat Anti-Aconitase 2, Biotinlyated Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09857-B 1
Biotinylated EB09857 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACO2, aconitase 2, ACONM, HEL-S-284, ICRD, OCA8, aconitase 2, mitochondrial, citrate hydro-lyase, epididymis secretory sperm binding protein Li 284, mitochondrial aconitase
Official Symbol: ACO2
Accession Number(s): NP_001089.1
Human GeneID(s): 50
Non-Human GeneID(s): 11429 (mouse), 79250 (rat)

Immunogen

Peptide with sequence C-QHVDVSPTSQRLQ., from the internal region of the protein sequence according to NP_001089.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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