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Goat Anti-Adiponectin Receptor 1 Antibody - Trial (EB06718-T)

Related Products: EBP06718 - Immunizing Peptide, Goat Anti-Adiponectin Receptor 1 Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB06718-T Goat Anti-Adiponectin Receptor 1 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Pig, Dog, Cow $105.00 Elite

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TestShot
EB06718-T 1
EB06718 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TESBP1A, FLJ42464, FLJ25385, CGI45, CGI-45 protein, PAQR1, CGI-45, ACDCR1, HGNC:24040, ADIPOR1, adiponectin receptor 1
Official Symbol: ADIPOR1
Accession Number(s): NP_057083.2; NP_001121159.1
Human GeneID(s): 51094
Important Comments: Variants (NP_057083.2; NP_001121159.1) encode the same protein.

Immunogen

Peptide with sequence RYGLEGGCTDDTLL, from the C Terminus of the protein sequence according to NP_057083.2; NP_001121159.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38kDa band observed in Human Skeletal Muscle lysates (calculated MW of 42.6kDa according to NP_057083.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Thrush AB, Heigenhauser GJ, Mullen KL, Wright DC, Dyck DJ.
Palmitate acutely induces insulin resistance in isolated muscle from obese but not lean humans.
Am J Physiol Regul Integr Comp Physiol. 2008 Apr;294(4):R1205-12.
PMID: 18305020

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