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Goat Anti-Androgen Receptor Antibody - Trial (EB06441-T)

Code Name Applications Availability Size Tested species Price Grade  
EB06441-T Goat Anti-Androgen Receptor Antibody - Trial Pep-ELISA, WB, IHC, IF, ChIP Yes 20µg specific antibody in 40µl Human $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

TestShot
EB06441-T 1
EB06441(0.5µg/ml) staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06441-T 2
EB06441 (2µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06441-T 3
EB06441 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06441-T 4
EB06441 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06441-T 5
ChIP of 2ug EB06441 with 1ug DHT-treated HEC50 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.
Click here to download PDF datasheet.

Target Protein

Principal Names: androgen receptor, dihydrotestosterone receptor, AR, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
Official Symbol: AR
Accession Number(s): NP_000035.2
Human GeneID(s): 367
Non-Human GeneID(s): 11835 (mouse), 24208 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Immunogen

Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/ml.

Immunofluorescence: Strong expression of the protein seen in the mitochondrial/cytoplasmic of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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