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Goat Anti-ARPC2 Antibody - Trial (EB05433-T)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05433-T Goat Anti-ARPC2 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Dog, Cow $105.00 Elite

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TestShot
EB05433-T 1
EB05433 (3.8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05433-T 2
EB05433 staining (0.2µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: actin related protein 2/3 complex subunit 2, ARP2/3 protein compex subunit 34, PNAS-139, p34-Arc, PRO2446, ARC34, actin related protein 2/3 complex, subunit 2, 34kDa, ARPC2
Official Symbol: ARPC2
Accession Number(s): NP_005722.1; NP_690601.1
Human GeneID(s): 10109
Non-Human GeneID(s): 76709 (mouse)
Important Comments: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Immunogen

Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1; NP_690601.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5µg/ml.
IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Li Y, Grenklo S, Higgins T, Karlsson R.
The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails.
Eur J Cell Biol. 2008 Nov;87(11):893-904.
PMID: 18707793

The goat polyclonal antibody used in this paper was manufactured by us:
Fougner SL, Bollerslev J, Latif F, Hald JK, Lund T, Ramm-Pettersen J, Berg JP.
Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment.
J Clin Endocrinol Metab. 2008 Apr;93(4):1211-6.
PMID: 18230656

The goat polyclonal antibody used in this paper was manufactured by us:
Sakata D, Taniguchi H, Yasuda S, Adachi-Morishima A, Hamazaki Y, Nakayama R, Miki T, Minato N, Narumiya S.
Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1.
J Exp Med. 2007 Sep 3;204(9):2031-8.
PMID: 17682067

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