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Goat Anti-ASC / TMS1 Antibody - Trial (EB05291-T)

Related Products: EBP05291 - Immunizing Peptide, Goat Anti-ASC / TMS1 Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05291-T Goat Anti-ASC / TMS1 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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TestShot
EB05291-T 1
EB05291 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05291-T 2
HEK293 overexpressing ASC (RC215592) and probed with EB05291 (mock transfection in first lane).
EB05291-T 3
EB05291 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TMS-1, target of methylation-induced silencing-1, caspase recruitment domain protein 5, apoptosis-associated speck-like protein containing a CARD, TMS, MGC10332, CARD5, PYD and CARD domain containing, TMS1, ASC, PYCARD
Official Symbol: PYCARD
Accession Number(s): NP_037390.2; NP_660183.1
Human GeneID(s): 29108
Important Comments: This antibody is expected to recognise all reported Human isoforms according to NP_037390.2 and NP_660183.1.

Immunogen

Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2; NP_660183.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 22kDa band observed in Hela and Human Kidney lysates (predicted MW of 22kDa according to NP_037390.2). Recommended for use at 1-3µg/ml.
IHC: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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