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Goat Anti-Aurora kinase A / AURKA Antibody - Trial (EB05035-T)

Related Products: EBP05035 - Immunizing Peptide, Goat Anti-Aurora kinase A / AURKA Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05035-T Goat Anti-Aurora kinase A / AURKA Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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TestShot
EB05035-T 1
EB05035 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05035-T 2
HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB05035 in the right panel (vector-only transfection in first and fourth lanes).
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000166071, OTTHUMP00000031345, OTTHUMP00000031344, OTTHUMP00000031343, OTTHUMP00000031342, OTTHUMP00000031340, serine/threonine protein kinase 6, serine/threonine protein kinase 15, serine/threonine kinase 6, serine/threonine kinase 15, breast-tumor-amplified kinase, aurora/IPL1-like kinase, aurora-related kinase 1, aurora-A, IPL1-related kinase, STK7, STK6, STK15, MGC34538, BTAK, AURORA2, AURA, ARK1, AIK, aurora kinase A, AURKA
Official Symbol: AURKA
Accession Number(s): NP_003591.2
Human GeneID(s): 6790
Important Comments: Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1

Immunogen

Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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