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Goat Anti-CBL Antibody - Trial (EB05288-T)

Related Products: EBP05288 - Immunizing Peptide, Goat Anti-CBL Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05288-T Goat Anti-CBL Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Dog, Pig, Cow $105.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
TestShot
EB05288-T 1
EB05288 staining (2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: oncogene CBL2, Cas-Br-M ecotropic retroviral transforming sequence (Oncogene CBL2), Cas-Br-M (murine) ecotropic retroviral transforming sequence, RNF55, C-CBL, CBL2, CBL
Official Symbol: CBL
Accession Number(s): NP_005179.2
Human GeneID(s): 867
Non-Human GeneID(s): 12402 (mouse)

Immunogen

Peptide with sequence C-REFVSISSPAHVAT, from the C Terminus of the protein sequence according to NP_005179.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 120kDa band seen in 3T3 lysate. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

The goat polyclonal antibody used in the following papers was manufactured by us:
Quinones GA, Jin J, Oro AE.
I-BAR protein antagonism of endocytosis mediates directional sensing during guided cell migration.
J Cell Biol. 2010 Apr 19;189(2):353-67.
PMID: 20385776

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