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Code Name Applications Availability Size Tested species Price Grade  
EB10236-B Goat Anti-CIRBP (aa 161-172), Biotinylated Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10236-B 1
Biotinylated EB10236 (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: CIRBP, cold inducible RNA binding protein, CIRP, A18 hnRNP, cold inducible RNA-binding protein, glycine-rich RNA binding protein, Cold-inducible RNA-binding protein
Official Symbol: CIRBP
Accession Number(s): NP_001271.1
Human GeneID(s): 1153
Non-Human GeneID(s): 12696 (mouse), 81825 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_001271.1) only.

Immunogen

Peptide with sequence C-RDSYDSYATHNE., from the C Terminus of the protein sequence according to NP_001271.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 18kDa band observed in Rat Testis lysates (calculated MW of 18.6kDa according to Rat NP_112409.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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