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Goat Anti-COX2 / PTGS2 Antibody - Trial (EB05286-T)

Related Products: EBP05286 - Immunizing Peptide, Goat Anti-COX2 / PTGS2 Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05286-T Goat Anti-COX2 / PTGS2 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Dog, Pig, Cow $105.00 Elite

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TestShot
EB05286-T 1
EB05286 staining (0.5µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: cyclooxygenase 2b, GRIPGHS, prostaglandin G/H synthase and cyclooxygenase, hCox-2, PGHS-2, PGG/HS, PHS-2, COX-2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX2, PTGS2
Official Symbol: PTGS2
Accession Number(s): NP_000954.1
Human GeneID(s): 5743

Immunogen

Peptide with sequence C-NPTVLLKERSTEL, from the C Terminus of the protein sequence according to NP_000954.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70-75kDa band observed in H460 lysates (predicted MW of 69kDa according to NP_000954.1). Recommended for use at 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Pisani LF, Antonini S, Pocar P, Ferrari S, Brevini TA, Rhind SM, Gandolfi F.
Effects of pre-mating nutrition on mRNA levels of developmentally relevant genes in sheep oocytes and granulosa cells.
Reproduction. 2008 Sep;136(3):303-12.
PMID: 18515315

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