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Goat Anti-Cytochrome P450 1A2 (mouse) Antibody (EB11171)

Code Name Applications Availability Size Tested species Price Grade  
EB11171 Goat Anti-Cytochrome P450 1A2 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11171 1
EB11171 (0.5µg/ml) staining of Human Liver (A) and (1ug/ml) Lung (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11171 2
EB11171 (1µg/ml) staining of fetal Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: aromatic compound inducible, CP12, Cyp1a1, CYPIA2, cytochrome P450 1A2, cytochrome P450 family 1 subfamily a polypeptide 1, cytochrome P450, 1a2, aromatic compound inducible, cytochrome P450, family 1, subfamily A, polypeptide 2, cytochrome P450-P2, cytochrome P450-P3, P450-3, Cyp1a2
Official Symbol: Cyp1a2
Accession Number(s): NP_034123.1
Non-Human GeneID(s): 13077 (mouse), 24297 (rat)
Important Comments: This antibody is not expected to cross-react to the similar P450 1A1.

Immunogen

Peptide with sequence C-HSENYKDNGGLIPEEK, from the internal region of the protein sequence according to NP_034123.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Mouse fetal Liver lysates and approx. 55kDa in Human Liver and Lung lysates (calculated MW of 58.4kDa according to Human NP_000752.2 and 58.2kDa according to Mouse NP_034123.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Mouse, Rat

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