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Code Name Applications Availability Size Tested species Price Grade  
EB12359-B Goat Anti-cytokeratin 19 (aa285-298), Biotinylated Antibody Pep-ELISA, WB, EIA Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12359-B 1
Biotinylated EB12359 (0.3µg/ml) staining of Human Colorectal cancer lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: KRT19, keratin 19, type I, CK19, K19, K1CS, 40-kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I, 40-kd, keratin-19, keratin, type I cytoskeletal 19
Official Symbol: KRT19
Accession Number(s): NP_002267.2
Human GeneID(s): 3880

Immunogen

Peptide with sequence C-HTEQLQMSRSEVTD., from the internal region of the protein sequence according to NP_002267.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in Human Colorectal cancer lysates (calculated MW of 44.1kDa according to NP_002267.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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