Change country
Home
Code Name Applications Availability Size Tested species Price Grade  
EB06377-B Goat Anti-GAPDH (C Terminus) Loading Control, biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06377-B 1
Biotinylated EB06377 (0.5µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HEL-S-162eP, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH, HGNC:4141, MGC88685, glyceraldehyde 3-phosphate dehydrogenase
Official Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Immunogen

Peptide with sequence C-HQVVSSDFNSDT., from the C Terminus of the protein sequence according to NP_002037.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Dog, Pig

Product Reviews

Please login to review this product.