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Goat Anti-Klk6 / neurosin (mouse) Antibody (EB09307)

Related Products: EBP09307 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09307 Goat Anti-Klk6 / neurosin (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09307 1
EB09307 staining (2µg/ml) of Mouse Liver lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Klk6, kallikrein related-peptidase 6, AI849898, Bssp, Klk29, Klk7, Prss18, Prss9, neurosin, kallikrein 29, kallikrein 6, kallikrein 7, protease M, protease, serine, 18, protease, serine, 9
Official Symbol: Klk6
Accession Number(s): NP_035307.1
Non-Human GeneID(s): 19144 (mouse), 29245 (rat)

Immunogen

Peptide with sequence KEKPGVYTDVCTHIR, from the C Terminus of the protein sequence according to NP_035307.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 22kDa band observed in Mouse Liver lysates (calculated MW of 27.5kDa according to NP_035307.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 48kDa. Both bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat, Cow

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