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|EB05444||Goat Anti-Melanophilin (mouse) Antibody||Pep-ELISA, WB, ICC, IF, GST-pull down||100µg specific antibody in 200µl||Mouse||$315.00|
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Peptide with sequence C-ARHIFAKPVMAQQP, from the C Terminus of the protein sequence according to NP_443748.2.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. See also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
GST-pull down assay: See publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
Singh RK, Mizuno K, Wasmeier C, Wavre-Shapton ST, Recchi C, Catz SD, Futter C, Tolmachova T, Hume AN, Seabra MC.
Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion.
FEBS J. 2013 Feb;280(3):892-903.
This antibody has been successfully used (in WB, IF on Mouse melanocytes) in the following paper:
Figueiredo AC, Wasmeier C, Tarafder AK, Ramalho JS, Baron RA, Seabra MC.
Rab3GEP Is the Non-redundant Guanine Nucleotide Exchange Factor for Rab27a in Melanocytes.
J Biol Chem. 2008 Aug 22;283(34):23209-16.
This antibody has been successfully used in the following paper:
Hume AN, Tarafder AK, Ramalho JS, Sviderskaya EV, Seabra MC.
A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes.
Mol Biol Cell. 2006 Nov;17(11):4720-35.