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Goat Anti-MMP9 Antibody (EB06890)

Code Name Applications Availability Size Tested species Price Grade  
EB06890 Goat Anti-MMP9 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06890 1
EB06890 (1µg/ml) staining of Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06890 2
EB06890 (0.1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MMP9, matrix metalloproteinase 9, gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase, HGNC:7176, CLG4B, GELB, 92kD type IV collagenase, gelatinaseB, macrophage gelatinase, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase), type V collagenase
Official Symbol: MMP9
Accession Number(s): NP_004985.2
Human GeneID(s): 4318

Immunogen

Peptide with sequence C-KLDSVFEEPLSKK, from the internal region of the protein sequence according to NP_004985.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx.80kDa and 70kDa bands observed in Human Peripheral Blood Mononucleocytes lysates. These bands correspond to the mature forms (http://www.uniprot.org/uniprot/P14780). This protein has a calculated MW of 78.5kDa according to NP_004985.2, but the precursor is usually seen as 92kDa or larger. Recommended concentration: 1-3µg/ml. Approx.75kDa band observed in Mouse Heart lysates (calculated MW of 80.5kDa according to Mouse NP_038627.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human

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