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Code Name Applications Availability Size Tested species Price Grade  
EB07441 Goat Anti-NOTCH1 Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07441 1
EB07441 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: NOTCH1, Notch homolog 1, translocation-associated (Drosophila), TAN1, hN1, Notch (Drosophila) homolog 1 (translocation-associated), neurogenic locus notch homolog protein 1, notch1, translocation-associated notch protein TAN-1
Official Symbol: NOTCH1
Accession Number(s): NP_060087.2
Human GeneID(s): 4851

Immunogen

Peptide with sequence C-DLKARRKKSQDGK, from the internal region of the protein sequence according to NP_060087.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.

Immunofluorescence :Strong expression of the protein seen in the nucleus of U2OS cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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