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Goat Anti-NUMB Antibody - Trial (EB05296-T)

Code Name Applications Availability Size Tested species Price Grade  
EB05296-T Goat Anti-NUMB Antibody - Trial Pep-ELISA, IHC, IF, FC Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

TestShot
EB05296-T 1
EB05296 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05296-T 2
EB05296 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05296-T 3
EB05296 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05296-T 4
EB05296 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05296-T 5
EB05296 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: NUMB, numb homolog (Drosophila), S171, numb homolog
Official Symbol: NUMB
Accession Number(s): NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1
Human GeneID(s): 8650
Non-Human GeneID(s): 18222 (mouse)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.

Immunogen

Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 6-8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Cow

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