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Goat Anti-P19ARF (mouse) Antibody (EB11213)

Related Products: EBP11213 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11213 Goat Anti-P19ARF (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11213 1
EB11213 (0.3µg/ml) staining of Mouse (A) and Rat (B) Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Arf, ARF-INK4a, CDK4I, cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, Ink4a/Arf, INK4a-ARF, mitochondrial smARF, MTS1, OTTMUSP00000008143, p16, p16(INK4a), p16-INK4, p16INK4a, p16-INK4a, p19ARF, Pctr1, RP23-166I8.5, Cdkn2a
Official Symbol: Cdkn2a
Accession Number(s): NP_034007.1
Non-Human GeneID(s): 12578 (mouse)
Important Comments: This antibody is expected to recognize isoform p19ARF (NP_034007.1) only.

Immunogen

Peptide with sequence C-RRGPHRNPGPGDDD, from the internal region of the protein sequence according to NP_034007.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 18kDa band observed in Mouse and Rat Liver lysates (calculated MW of 19.2kDa according to Mouse NP_034007.1 and 17.4kDa according to Rat NP_113738.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse

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