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Goat Anti-Pecam1 / CD31 (mouse) Antibody (EB12605)

Code Name Applications Availability Size Tested species Price Grade  
EB12605 Goat Anti-Pecam1 / CD31 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $315.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12605 1
EB12605 (0.5µg/ml) staining of Mouse (A) and Rat (B) Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Pecam1, platelet/endothelial cell adhesion molecule 1, C85791, Cd31, PECAM-1, Pecam, PECAM-1/CD31, platelet endothelial cell adhesion molecule
Official Symbol: Pecam1
Accession Number(s): NP_032842.2; NP_001027550.1
Non-Human GeneID(s): 18613 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_032842.2; NP_001027550.1).

Immunogen

Peptide with sequence C-EKTTIEYEVKVH, from the internal region of the protein sequence according to NP_032842.2; NP_001027550.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80-85kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 80kDa according to Mouse NP_032842.2 and 76.2kDa according to Rat NP_113779.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse

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