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Code Name Applications Availability Size Tested species Price Grade  
EB07312 Goat Anti-PTCH (Internal) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB07312 1
EB07312 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1, PTCH protein +12b, PTCH protein +4\1, PTCH protein -10, patched, patched (Drosophila) homolog
Official Symbol: PTCH
Accession Number(s): NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1
Human GeneID(s): 5727
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).

Immunogen

Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific Reference

This antibody was successfully used in IHC in Human:
Gerardo Valadez J, Grover VK, Carter MD, Calcutt MW, Abiria SA, Lundberg CJ, Williams TV, Cooper MK.
Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation.
Cancer Lett. 2013 Jan 28;328(2):297-306.
PMID: 23063752

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