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Goat Anti-SM22 Alpha / Transgelin Antibody - Trial (EB06281-T)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06281-T | Goat Anti-SM22 Alpha / Transgelin Antibody - Trial | Pep-ELISA, WB |  |
20µg specific antibody in 40µl | Human, Mouse, Rat, Dog, Cow | $105.00 |  |
|
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$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: transgelin variant 2, DKFZp686B01212, TAGLN1, DKFZp686P11128, SM22-alpha, transgelin, WS3-10, SMCC, SM22, TAGLNOfficial Symbol: TAGLN
Accession Number(s): NP_003177.2
Human GeneID(s): 6876
Non-Human GeneID(s): 21345 (mouse), 25123 (rat)
Important Comments: NP_003177.2 and NP_001001522.1 represent identical protein. The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.
Immunogen
Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 23kDa bands observed in Human HeLa lysates and in lysates of Rat Colon (calculated MW of 22.6kDa according to NP_003177.2). Recommended concentration: 0.01-0.03µg/ml.
Species Reactivity
Tested: Human, Mouse, Rat, RabbitExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific References
This antibody has been successfully used in the following papers:
Donati C, Marseglia G, Magi A, Serratì S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P.
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for
GATA6.
PLoS One. 2011;6(5):e20389.
PMID: 21629665
Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P.
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.
Mol Biol Cell. 2010 Mar 15;21(6):1111-24.
PMID: 20089836
Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P.
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.
Cell Mol Life Sci. 2009 May;66(10):1741-54.
PMID: 19337690
Donoghue P, Doran P, Wynne K, Pedersen K Dunn MJ, Ohlendieck K.
Proteomic profiling of chronic low-frequency stimulated fast muscle.
Proteomics. 2007 Sep;7(18):3417-30.
PMID: 17708595
The goat polyclonal antibody used in the following papers was manufactured by us:
Léguillette R, Laviolette M, Bergeron C, Zitouni N, Kogut P, Solway J, Kachmar L, Hamid Q, Lauzon AM.
Myosin, transgelin, and myosin light chain kinase: expression and function in asthma.
Am J Respir Crit Care Med. 2009 Feb 1;179(3):194-204.
PMID: 19011151
Cohen ED, Ihida-Stansbury K, Lu MM, Panettieri RA, Jones PL, Morrisey EE.
Wnt signaling regulates smooth muscle precursor development in the mouse lung via a tenascin C/PDGFR pathway.
J Clin Invest. 2009 Sep;119(9):2538-49.
PMID: 19690384
High FA, Lu MM, Pear WS, Loomes KM, Kaestner KH, Epstein JA.
Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development.
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1955-9.
PMID: 18245384
Huang J, Cheng L, Li J, Chen M, Zhou D, Lu MM, Proweller A, Epstein JA, Parmacek MS.
Myocardin regulates expression of contractile genes in smooth muscle cells and is required for closure of the ductus arteriosus in mice.
J Clin Invest. 2008 Feb;118(2):515-25.
PMID: 18188448
Maile LA, Capps BE, Ling Y, Xi G, Clemmons DR.
Hyperglycemia alters the responsiveness of smooth muscle cells to insulin-like growth factor-I.
Endocrinology. 2007 May;148(5):2435-43.
PMID: 17255202
Ross JJ, Hong Z, Willenbring B, Zeng L, Isenberg B, Lee EH, Reyes M, Keirstead SA, Weir EK, Tranquillo RT, Verfaillie CM.
Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells.
J Clin Invest. 2006 Dec;116(12):3139-49.
PMID: 17099777
Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, Zhang J, Verfaillie C.
Multipotent adult progenitor cells from swine bone marrow.
Stem Cells. 2006 Nov;24(11):2355-66.
PMID: 16931778
Bandapalli OR, Geheeb M, Kobelt D, Kuehnle K, Elezkurtaj S, Herrmann J, Gressner AM, Weiskirchen R, Beule D, Blüthgen N, Herzel H, Franke C, Brand K.
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases.
Int J Cancer. 2006 Jan 1;118(1):74-89.
PMID: 16080196
Deaton RA, Su C, Valencia TG, Grant SR.
Transforming growth factor-beta1-induced expression of smooth muscle marker genes involves activation of PKN and p38 MAPK.
J Biol Chem. 2005 Sep 2;280(35):31172-81.
PMID: 15980430
Illi B, Scopece A, Nanni S, Farsetti A, Morgante L, Biglioli P, Capogrossi MC, Gaetano C.
Epigenetic histone modification and cardiovascular lineage programming in mouse embryonic stem cells exposed to laminar shear stress.
Circ Res. 2005 Mar 18;96(5):501-8.
PMID: 15705964