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Goat Anti-VAV2 Antibody - Trial (EB05337-T)

Code Name Applications Availability Size Tested species Price Grade  
EB05337-T Goat Anti-VAV2 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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EB05337-T 1
EB05337 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05337-T 2
EB05337 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2
Official Symbol: VAV2
Accession Number(s): NP_001127870.1; NP_003362.2
Human GeneID(s): 7410
Non-Human GeneID(s): 22325 (mouse)
Important Comments: This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).


Peptide with sequence ETEAKYYRTLEDIEC, from the internal region of the protein sequence according to NP_001127870.1; NP_003362.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5µg/ml.
IHC: Paraffin embedded Human Colon. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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