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Rabbit anti-goat IgG antibody AP Conjugated (EB2ND-002-AP)

Code Name Applications Availability Size Tested species Price Grade  
EB2ND-002-AP Rabbit anti-goat IgG antibody AP Conjugated Yes 1mg $165.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

Description

Alkaline Phosphatase conjugated and affinity purified anti-goat IgG (H+L) (Rabbit) with minimum cross-reactivity to serum proteins of Human, Mouse and Rat.

Preparation

This product was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, goat IgG and goat serum.  It was conjugated to Alkaline Phosphatse according to a modified procedure from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971. Stabiliser: 10mg/ml Bovine Serum Albumin (BSA), IgG and protease-free. Preservative: 0.1% (w/v) Sodium Azide.

Storage and Handling

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Applications

Suitable for immunoblot (western or dotblot), ELISA, and other peroxidase-based immunoassays. Recommended dilutions:

ELISA: 1:2,000 -1:10,000  WB: 1:500 - 1: 2,500 IHC: 1:200 - 1:1,000

 

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