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SP1003 Affinity Purified anti-Phosphopeptide Polyclonal Antibody

Phosphopeptide antibody purification package - Introductory offer - $1950 (including shipping to Europe and USA)

Includes:
Delivered:
Time Frame:
  • Peptide synthesis ( >70% purity, 15 mg) of phosphopeptide and the corresponding
    unphosphorylated peptide
  • Conjugation of phosphopeptide to hemocyanin carrier
  • Immunization of 2 goats (11 week protocol)
  • Terminal Bleeds – expect between 500ml and 1000ml per goat (note only purified antibody will be delivered)
  • Affinity purification of phospho-specific antibody from each serum giving good responses by ELISA. Affinity purification column using the phosphopeptide and an affinity depletion column using the nonphosphopeptide.
  • ELISA test on both phosphorylated and non-phosphorylated peptides.

 

 

  • ELISA results
  • 3 mg of unconjugated peptides, MS and HPLC reports
  • affinity purified antibody from each goat

 

 

 

 

 

 

  • Peptide Synthesis: 2-3 weeks
  • Immunization and ELISA: 13 weeks
  • Purification and Shipping: 2-3 weeks
  • Total 17-19 weeks

 

 

 

 

 

 

 

 

 Regular custom orders can be submitted online here. If you wish to download an order form, please click here. Or click here to view our polyclonal antibody and custom antibody services.