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SP1004 Affinity Purified anti-Modified Peptide Polyclonal Antibody

 

Modified peptide-specific antibody purification package - Price depending on the peptide modification*

Includes:
Delivered:
Time Frame:
  • Peptide synthesis ( >70% purity, 15 mg) of custom modified peptide and the corresponding 
    unmodified peptide
  • Conjugation of modified peptide to hemocyanin carrier
  • Immunization of 2 goats (11 week protocol)
  • Terminal Bleeds – expect between 500ml and 1000ml per goat (note only purified antibody will be delivered)
  • Initial antigen affinity purification of modified peptide-specific antibody from each serum giving good responses by ELISA. Affinity purification column using the modified peptide and an affinity depletion column using the nonmodified peptide.
  • ELISA test on both modified peptide and non-modified peptide.

 

 

 
  • ELISA results
  • 3 mg of unconjugated peptides, MS and HPLC reports
  • affinity purified antibody from each goat

 

 

 

 

 

 

 
  • Peptide Synthesis: 2-3 weeks
  • Immunization and ELISA: 13 weeks
  • Purification and Shipping: 2-3 weeks
  • Total 17-19 weeks

 

 

 

 

 

 

 

 

* Any modification to any residue in the immunizing peptide first needs to be discussed with the technical support ([email protected]), to check availability and price before ordering. Please click here to view our custom antibody and polyclonald antibody services.