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The success story of our promotion custom/collaboration service

In November 2011 we launched a program in order to assist scientists with a tight budget with our highly cost-effective antibody pipeline. For the price of one or two catalogue antibodies we developed two novel antibodies to the protein of the scientist’s interest and the scientist would provide the validating data to be used for marketing purposes. The reward would be up to 20 commercial units per validated antibody. We encourage academia to make use of this promotion as it provides the most cost-effective way of obtaining large quantities of anitbodies for a very low price and risk.

We occupy a special niche of manufacturing mono-epitopic polyclonal antibodies as a cost-effective alternative to monoclonal antibodies. This is achieved by using 10-15 residue peptides as the antigen and using goat as the host species. By affinity purification using the immunizing peptide we obtain high yields of antibodies with high affinity. Thus we provide high performance mono-specific antibodies and by buying from us in bulk the customer evades batch-to-batch variations (particularly a problem to assay developers). 

We had many successful returns of validating data generated by the applicants. They have been included in the product sheets (with acknowledgement) and the scientist got rewarded with 20 units of the antibody they requested.  Below is a compilation of products with the returned data that made this promotion so successful. 

For antibodies to non-human and non-rodent proteins we offer our regular custom service at world-beating rates! 

EB11418, RRAD (aa36-48):

a): EB11418 (0.5µg/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10µg protein in RIPA buffer) and compared with an HA-specific antibody. B): EB11418 (0.5µg/ml) staining of WT and KO lysates of Mouse Heart (100µg protein in RIPA buffer). Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA. Primary incubation was 1 hour. Detected by chemiluminescence. 

 

EB11473, Nalp10 / Nlrp10 (mouse):

EB11473 (1µg/ml) staining of Mouse Embryonic Fibroblasts lysate (25µg protein in RIPA buffer) and in HEK293 overexpressing Mouse Nalp10 (lane 3), Human NALP10 (lane 2) and empty vector (lane 1). . Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Dr. Thomas Kufer, University of Cologne, Germany.

EB11473 (5ug/ml) staining of Mouse Embryonic Fibroblasts (red), and of HeLa overexpressing Mouse Nalp10. Nuclear staining by DAPI (blue). Data provided by Dr. Thomas Kufer, University of Cologne, Germany.

EB11481, BPIFA1 / PLUNC:

EB11481 (2µg/ml) staining of secretions from Human primary airway cells in culture (lanes 1 and 2), and in Human Bronchoalveolar Lavage fluid (lanes 3 and 4) . Data obtained from Dr. C Bingle, AURM, University of Sheffield, UK. Primary incubation was 1 hour. Detected by chemiluminescence.

 

EB11536, MEX3C (aa541-554):

EB11536 (0.2µg/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35µg protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Primary incubation was 1 hour. Detected by chemiluminescence.

 

EB11540, RIF1:

EB11540 (1µg/ml) staining of HeLa and Mouse D3 (ES) lysates (35µg protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence.

 

EB11598, ZNRF1 (mouse):

N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with EB11598 (1µg/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

EB11598 (10µg/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).

 

EB11768, Contactin 4 / Big-2 (mouse aa160-172):

EB11768 (0.5µg/ml) staining of Mouse Olfactory bulb (lanes1 and 3) and Cerebral cortex (lanes 2 and 4), comparing wildtype (lanes 3 and 4) with KO mice (lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Gerrald Lodewijk and Peter Burbach, Rudolf Magnus Institute, Utrecht, Netherlands.

 

EB11876, Cd97 (mouse):

EB11876 (1µg/ml) staining of Mouse Colon (wt, left lane, knock-out right lane) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by infrared fluorescence (Odyssey). Data obtained from anonymous customer

EB11926, ERK3 / MAPK6:

HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with EB11926 (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.

 

EB11926 (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).

 

EB11927 and EB11928, MK5 / MAPKAPK5:

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with EB11927 (0.5ug/ml) in right panel and with EB11928 (0.5ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.

EB11927 and EB11928 (1.5ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).

 

EB11929 and EB11930, MK2 / MAPKAPK2:

EB11929 (0.5µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.

HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5ug/ml) in the left panel and with EB11930 (0.5ug/ml) in the right panel.

EB11929 and EB11930 (1.5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.

 

EB12006, CCDC3:

 

HEK293 overexpressing Human CCDC3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB12006 (0.5ug/ml) in the right panel (empty vector transfection in first lanes). Data obtained from Dr. YangXin Fu, Dept Oncology, University of Alberta, Edmonton, Canada.

 

EB12182, Ferd3l (aa56-68)



EB12182 (1µg/ml) staining of PFA-perfused cryosection of Mouse embryo E13.5. Primary incubation overnight at 4C. Alexa Fluor 488 detection (green) in combination with nuclear DAPI staining (blue). Data obtained by Ben Jerry Gonzales from Nissim Ben-Arie’s lab, Dept. of Cell and Developmental Biology, The Hebrew University of Jeruzalem, Israel.