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Code Name Applications Availability Size Tested species Price Grade  
EB12552 Goat Anti-CD74 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB12552 1
EB12552 (0.03µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, gamma chain of class II antigens
Official Symbol: CD74
Accession Number(s): NP_001020330.1; NP_004346.1
Human GeneID(s): 972
Important Comments: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).

Immunogen

Peptide with sequence C-HSLEQKPTDAPPK, from the internal region of the protein sequence according to NP_001020330.1; NP_004346.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 33kDa band observed in Human Tonsil lysates (calculated MW of 33.5kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB: Meza-Romero R et al (2019). PMID: 30353480.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot:
Meza-Romero R, Benedek G, Gerstner G, Kent G, Nguyen H, Offner H, Vandenbark AA
Increased CD74 binding and EAE treatment efficacy of a modified DRα1 molecular construct.
Metab Brain Dis. 2019 Feb;34(1):153-164
PMID: 30353480

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