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Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody (EB08135)

Code Name Applications Availability Size Tested species Price Grade  
EB08135 Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08135 1
EB08135 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08135 2
EB08135 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08135 3
EB08135 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB08135 4
EB08135 (2.5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08135 5
EB08135 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2
Official Symbol: EPB41L2
Accession Number(s): NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1
Human GeneID(s): 2037
Important Comments: This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1 (iso i), NP_001337243.1 (iso j), NP_001337249.1 (iso k).

Immunogen

Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary testing showed bands at approx 110+40kDa in Human Cerebellum lysatesafter 0.1µg/ml antibody staining (calculated MW of 113kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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