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Goat Anti-GAD25 / GAD67 Antibody (EB11071)

Related Products: EBP09109 - Immunizing Peptide, EBP11071 - Immunizing Peptide, Goat Anti-GAD1 (isoform GAD67) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11071 Goat Anti-GAD25 / GAD67 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11071 1
EB11071 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: 67 kDa glutamic acid decarboxylase, FLJ45882, GAD, GAD-67, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kDa), glutamate decarboxylase 67 kDa isoform, OTTHUMP00000204990, OTTHUMP00000204993, OTTHUMP00000204994, OTTHUMP00000204995, SCP, GAD1
Official Symbol: GAD1
Accession Number(s): NP_038473.2; NP_000808.2
Human GeneID(s): 2571
Important Comments: This antibody is expected to recognize both reported isoforms (NP_038473.2; NP_000808.2).

Immunogen

Peptide with sequence C-QRTNSLEEKSR, from the internal region of the protein sequence according to NP_038473.2; NP_000808.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow

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