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Goat Anti-HSD11B1 / HDL Antibody (EB06597)

Related Products: EBP06597 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06597 Goat Anti-HSD11B1 / HDL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06597 1
EB06597 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HSD11B1, HDL, 11-DH, HSD11L, MGC13539, 11-beta-HSD1, hydroxysteroid (11-beta) dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1
Official Symbol: HSD11B1
Accession Number(s): NP_005516.1; NP_861420.1
Human GeneID(s): 3290
Important Comments: This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.

Immunogen

Peptide with sequence CTSYNMDRFINK, from the C Terminus of the protein sequence according to NP_005516.1; NP_861420.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 35kDa band observed in Human Liver lysates (calculated MW of 32.4kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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