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Goat Anti-LITAF Antibody (EB08099)

Code Name Applications Availability Size Tested species Price Grade  
EB08099 Goat Anti-LITAF Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08099 1
EB08099 (6.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LITAF, lipopolysaccharide-induced TNF factor, CMT1C, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7, LPS-induced TNF-alpha factor, small integral membrane protein of lysosome/late endosome, tumor protein
Official Symbol: LITAF
Accession Number(s): NP_004853.2; NP_001129945.1
Human GeneID(s): 9516
Non-Human GeneID(s): 56722 (mouse), 65161 (rat)
Important Comments: This antibody is excpected to crossreact with both reported isoforms (NP_004853.2; NP_001129945.1).

Immunogen

Peptide with sequence C-PDGKGMNPPSYYTQ, from the internal region of the protein sequence according to NP_004853.2; NP_001129945.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 25kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 6.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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