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Code Name Applications Availability Size Tested species Price Grade  
EB08990 Goat Anti-OXTR Antibody Pep-ELISA, WB, IHC Contact sales for delivery estimate 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613

EB08990 1
EB08990 (0.5µg/ml) staining of Rat Uterus (U) and Rat Skeletal Muscle (M) lysates (35µg protein in RIPA buffer). Data obtained from Maree Gould, University of Otago, New Zealand. Detected by chemiluminescence.
EB08990 2
EB08990 (2µg/ml) staining of paraffin embedded Human Prostate Cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB08990 3
EB08990 (5µg/ml) staining of Human Prostate stromal cells. Overnight incubation at 4C and detected with Alexafluor secondary conjugated antibody. This data is from a previous batch, not on sale.
EB08990 4
EB08990 (5µg/ml) staining of PC3 cells. Detected by DAB. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: OXTR, oxytocin receptor, OT-R
Official Symbol: OXTR
Accession Number(s): NP_000907.2
Human GeneID(s): 5021


Peptide with sequence C-RRLGETSASKKSN, from the C Terminus of the protein sequence according to NP_000907.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Rat Uterus lysates, but none in Rat Skeletal Muscle lysates as expected (calculated MW of 42.9kDa according to Rat NP_037003.2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Whittington et al, Cell Tissue Res. 2004 Nov;318(2):375-82. PMID: 15459766). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Prostate Cancer shows staining of the stromal component of smooth muscle. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

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