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Goat Anti-P2rx4 (mouse) Antibody (EB11413)

Related Products: EBP08830 - Immunizing Peptide, EBP11413 - Immunizing Peptide, Goat Anti-P2X4 / P2X4R Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11413 Goat Anti-P2rx4 (mouse) Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11413 1
EB11413 (0.3µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11413 2
EB11413 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB11413 3
EB11413 (5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AI504491, ATP receptor, AW555605, D5Ertd444e, ionotropic purinergic receptor, OTTMUSP00000023741, OTTMUSP00000023745, OTTMUSP00000023746, OTTMUSP00000023747, P2X purinoceptor 4, P2X4, purinergic receptor P2X, ligand-gated ion channel, 4, P2rx4
Official Symbol: P2rx4
Accession Number(s): NP_035156.2
Human GeneID(s): 5025
Non-Human GeneID(s): 18438 (mouse), 29659 (rat)

Immunogen

Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of the protein sequence according to NP_035156.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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