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Goat Anti-Pericentrin 1 / NUP85 Antibody - Trial (EB06201-T)

Related Products: EBP06201 - Immunizing Peptide, Goat Anti-Pericentrin 1 / NUP85 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06201-T Goat Anti-Pericentrin 1 / NUP85 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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ARP American Research Products, Inc
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TestShot
EB06201-T 1
EB06201 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85kDa, FLJ12549, Nup75, nucleoporin 85
Official Symbol: NUP85
Accession Number(s): NP_079120.1; NP_001290205.1; NP_001317401.1
Human GeneID(s): 79902
Non-Human GeneID(s): 18541 (mouse)
Important Comments: This antibody is expected to recognise al reported isoforms.

Immunogen

Peptide with sequence C-ARAIIREGSLEGS, from the C Terminus of the protein sequence according to NP_079120.1; NP_001290205.1; NP_001317401.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used in IHC on Rat:
Toyo Ikebukuro et al.
Disulfiram Ophthalmic Solution Inhibited Macrophage Infiltration by Suppressing Macrophage Pseudopodia Formation in a Rat Corneal Alkali Burn Model
Int. J. Mol. Sci. 2023, 24(1), 735; https://doi.org/10.3390/ijms24010735
PMID: 0

This antibody has been successfully used in WB and IP on Human:
Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K.
FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.
J Immunol. 2009 Nov 15;183(10):6387-94.
PMID: 19841162

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