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Goat Anti-Psoriasin / S100A7 Antibody (EB07277)

Code Name Applications Availability Size Tested species Price Grade  
EB07277 Goat Anti-Psoriasin / S100A7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07277 1
EB07277 (0.1µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07277 2
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with EB07277 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB07277 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB07277 3
EB07277 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: S100A7, S100 calcium binding protein A7 (psoriasin 1), HGNC:10497, PSOR1, S100A7c, S100 calcium-binding protein A7, S100 calcium-binding protein A7 (psoriasin 1), psoriasin 1, Psoriasin
Official Symbol: S100A7
Accession Number(s): NP_002954.1
Human GeneID(s): 6278

Immunogen

Peptide with sequence C-HKYTRRDDKIDKP, from the internal region of the protein sequence according to NP_002954.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 15kDa band observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 1-3µg/ml. In transfected HEK293 transiently expressing full-length Human S100A7 (myc and DYKDDDDK tagged), a band of approx. 16kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5µg/ml. Approx 15kDa band was also observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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