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Code Name Applications Availability Size Tested species Price Grade  
EB10878 Goat Anti-TXNDC5 (aa269-282) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10878 1
EB10878 (0.05µg/ml) staining of Human Lymph Nodes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10878 2
EB10878 (0.1µg/ml) staining of Mouse Lymph Nodes (A), Stomach (B) and Intestine (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10878 3
EB10878 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TXNDC5, thioredoxin domain containing 5 (endoplasmic reticulum), ERP46, EndoPDI, Hcc-2, MGC3178, PDIA15, UNQ364, ER protein 46, endoplasmic reticulum resident protein 46, endothelial protein disulphide isomerase, endoplasmic reticulum protein ERp46, protein disulfide isomerase family A, member 15, thioredoxin domain-containing protein 5, thioredoxin related protein, thioredoxin-like protein p46
Official Symbol: TXNDC5
Accession Number(s): NP_110437.2; NP_001139021.1
Human GeneID(s): 81567
Non-Human GeneID(s): 105245 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).

Immunogen

Peptide with sequence C-RDGKKVDQYKGKRD, from the internal region of the protein sequence according to NP_110437.2; NP_001139021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50+38kDa bands observed in Human Lymph Nodes lysates (calculated MW of 47.6kDa according to NP_110437.2 and of 36.2kDa according to NP_001139021.1). A 48kDa band was observed in Mouse Lymph Node while a 37-38kDa doublet was observed n Mouse Stomach lysates and in Mouse Intestine lysates. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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