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Goat Anti-VE-cadherin (aa537-549) Antibody (EB12666)

Code Name Applications Availability Size Tested species Price Grade  
EB12666 Goat Anti-VE-cadherin (aa537-549) Antibody Pep-ELISA, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB12666 1
EB12666 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDH5, cadherin 5, type 2 (vascular endothelium), 7B4, CD144, 7B4 antigen, VE-cadherin, cadherin 5, type 2, VE-cadherin (vascular epithelium), cadherin-5, cd144 antigen, endothelial-specific cadherin, vascular endothelial cadherin
Official Symbol: CDH5
Accession Number(s): NP_001786.2
Human GeneID(s): 1003

Immunogen

Peptide with sequence C-TVKYGQFDREHTK, from the internal region of the protein sequence according to NP_001786.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 100kDa band in lysates of cell line HeLa and approx. 110kDa in A549 at 1-2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observed, given the calculated size of 87.5kDa according to NP_001786.2.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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