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Goat Anti-ABAD / HADH2 Antibody (EB06598)

Related Products: EBP06598 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06598 Goat Anti-ABAD / HADH2 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

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EB06598 1
EB06598 staining (0.3µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: type 10 17beta-hydroxysteroid dehydrogenase, AB-binding alcohol dehydrogenase, type 10 17b-HSD, hydroxyacyl-Coenzyme A dehydrogenase, type II, 17b-HSD10, HSD17B10, MHBD, ERAB, ABAD, HADH2
Official Symbol: HSD17B10
Accession Number(s): NP_004484.1; NP_001032900.1
Human GeneID(s): 3028
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484.1; NP_001032900.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2µg/ml. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Tilton RG, Haidacher SJ, Lejeune WS, Zhang X, Zhao Y, Kurosky A, Brasier AR, Denner L.
Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry.
Proteomics. 2007 May;7(10):1729-42.
PMID: 17436268

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