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Contact
- Email [email protected]
- UK tel: 01869 238326
- US tel: 888-320-4628 (#2)
Protocols
- Western Blotting Protocol
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- Flow Cytometry Protocol NK cell assay
- Cell lysate preparation
- Elisa titre determination
- Tissue lysate preparation
- Blocking with the immunizing peptide
- IHC protocol for LSBio tested Paraffin sections
- ELISA Pair Testing Protocol
- MSDS - all goat antibodies
- MSDS - all peptides
Technical support
Frequently asked questions
Quality Control and Production
- What is the formulation of the antibodies?
- Is the amount of Sodium Azide present hazardous?
- How do I store my antibody?
- What is the isotype of the Everest antibodies?
- What is the species and location of the goats, Everest antibodies are raised in?
- What is the origin of your BSA serum?
- How do we dissolve the immunizing peptide and how do we mix it with the antibody?
- Are the datasheets correct and up-to-date?
- What is the difference between Tested and Expected species, and does the antibody cross-react to the species of my project?
- Does the antibody work in the application of my project?
- Why can I not reproduce the data as shown on the product sheet?
- The antibody shows high non-specific background. How do I reduce this background?
- What secondary antibody is recommended?
- What is the difference between Elite and Aspiring grades?
- Can the LSBio watermark be removed from IHC images?
- What does the C-/-C at the beginning/ending of the peptide sequences stand for?
Delivery and availability
- Can we obtain the immunizing peptide?
- Can we get BSA-free antibodies?
- Can we get pre-immune serum?
- Can I get a free antibody sample to try?
- Do you deliver antibodies in bulk?
- Do you have a distributor in my country?
- Does Everest provide custom service?
Shi-Fix and Shi-Fix Plus Coverslips
- What are the differences between Shi-Fix and Shi-Fix Plus?
- What is the shelf life of the Shi-Fix and Shi-Fix Plus coverslips?
- What is the thickness of the coverslips?
- It is possible to fix micro-organisms, free mitochondria, organelle membranes, and mouse embryos (with transparent bodies)?
- It is possible to measure the metabolic activity of the fixed cells?
- Could you disclose the composition of the coating material?
- Could you tell us about the data, advantages and features compared to CELL-TAK?
- Do you have a list of cells that you have successfully used with Shi-Fix?
- Which cell lines have been difficult to attach/ fix onto the Shi-Fix?
- Can we seed cells without washing with PBS?
What is the formulation of the antibodies?
All our un-conjugated antibodies are delivered as 100µg of purified antibody in 200µl buffer: 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin.
Is the amount of Sodium Azide present hazardous?
No. The tiny amount of sodium azide (NaN3) present in our products ensures that they comply with the regulations for the excepted quantity exemptions of dangerous goods. See the MSDS.
Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4C for a few weeks and still remain viable.
What is the isotype of the Everest antibodies?
All our goat antibodies are polyclonal and affinity-purified with the immunizing peptide. They are all IgG, and require Protein G and not Protein A, for purification.
What is the species and location of the goats, Everest antibodies are raised in?
Domesticated Nepalese goat, Capra hircus.
What is the origin of your BSA serum?
US origin, Bovine.
How do we dissolve the immunizing peptide and how do we mix it with the antibody?
Please refer to “Blocking with the immunizing peptide” protocol, listed on Tech Support page.
Are the datasheets correct and up-to-date?
Yes, the current batch on sale is on the website. We are continuously working to add additional species and applications to our products, and this information is added to our datasheets when QC is confirmed.
Tested species – species verified as positive by QC testing, citations, or customer feedback, as listed on our datasheets.
Expected species – immunogen peptide sequence shows 100% homology with species, according to NCBI Protein BLAST.
Does the antibody work in the application of my project?
All verified applications are shown on our datasheets – we cannot guarantee it will work in those not listed.
Why can I not reproduce the data as shown on the product sheet?
The Quality Control data as presented on the product datasheets are guaranteed. However, different tissue types or cell lines may result in different patterns. For any issues, please refer to relevant protocol listed on Tech Support page, or contact: [email protected].
The antibody shows high non-specific background. How do I reduce this background?
Please refer to “Western blot Trouble shooting guide” and “Western blot Guidelines” listed on the Tech Support page. Further discussion can be found by following link: non-specific background.
What secondary antibody is recommended?
We recommend our affinity purified IgG secondary antibody, which has minimal cross reactivity to human and rodent serum proteins. To purchase please follow the link: /category/secondary-antibodies/
What is the difference between Elite and Aspiring grades?
Please refer to the Products tab on Home page.
Can the LSBio watermark be removed from IHC images?
No. Under our current agreement with Lifespan, the watermark must remain, for these are products which are manufactured by Everest Biotech, but are IHC tested by them. We do not take responsibility for removal of the watermark.
What does the C-/-C at the beginning/ending of the peptide sequences stand for?
This means that a Cysteine has been added to the peptide sequence, with the sequence then either beginning or ending with C.
Can we obtain the immunizing peptide?
Yes. Related peptides can be found on each antibody datasheet.
Can we get BSA-free antibodies?
Yes. The minimum quantity required per order is 1 mg. Please email our Sales team for a quotation: [email protected].
No. We only sell purified antibody.
Can I get a free antibody sample to try?
We do not provide free antibodies. But all of our antibodies are covered by our 100% guarantee. If you are interested in purchasing a smaller sample, please contact: [email protected].
Do you deliver antibodies in bulk?
Yes. For pricing please click here.
Do you have distributor in my country?
To check for a distributor in your country, please refer to Distributor tab on Home page. If you can't find your country on the list, please email: [email protected].
Does Everest provide custom service?
No. Unfortunately, we no longer provide this service.
What are the differences between Shi-Fix and Shi-Fix Plus?
1. Size: Shi-Fix coverslips are 12mm in diameter, and the Shi-Fix Plus are 15mm.
2. Quality: The plus line is made with high quality German glass for high resolution imaging.
3. Culture maintenance: The plus line is suitable for culturing the suspension cells for a day or two prior to staining.
What is the shelf life of the Shi-Fix and Shix-Fix Plus coverslips?
The shelf life for both Shi-fix and Shi-fix plus is one year.
What is the thickness of the coverslips?
Shi-Fix coverslips (pack sizes of 25 + 50): ø 12mm x 0.17mm (#1.5); Shi-Fix Plus coverslips: ø 15mm x 0.17mm (#1.5). Therefore, the thickness is 0.17mm.
Yes, it is possible to fix micro-organisms, such as E.coli, free mitochondria and organelle membranes. However, mouse embryos have yet to be tested.
It is possible to measure the metabolic activity of the fixed cells?
Yes, it is possible to measure the metabolic activity of the fixed/ attached cells, as long as the cells are “living”.
Could you disclose the composition of the coating material?
We are unable to disclose the composition of the coating material, but it is a combination of mammalian protein and a synthetic organic compound.
Could you tell us about the data, advantages and features compared to CELL-TAK?
We haven’t yet compared Shi-Fix to CELL-TAK, so there is no data. However, our product has been successfully used in many publications and is now endorsed as a standard operating protocol in a Cancer institute of the National institutes of health, USA.
Do you have a list of cells that you have successfully used with Shi-Fix?
The list of cells that have successfully attached onto the Shifix are as follows: Jurkat cells, K562 cells, U937 cells, Daudi cells, PC12 cells, Raw264.7 cells, MOLT4 cells, PBL culture and Splenocytes.
Which cell lines have been difficult to attach/ fix onto the Shi-Fix?
PBL and K562 have relatively weak attachment on Shi-Fix coverslips. We have Shi-Fix plus coverslips for better attachment of these kind of difficult to attach cell lines.
Can we seed cells without washing with PBS?
Yes, it is possible to seed cells containing medium and there will be enough cells for microscopy. However, the attachment of cells containing medium will be less than that of cells containing PBS.