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Frequently asked questions
Quality Control and Production
Delivery and availability
Shi-Fix and Shi-Fix Plus Coverslips
All our un-conjugated antibodies are delivered as 100µg of purified antibody in 200µl buffer: 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin.
No. The tiny amount of sodium azide (NaN3) present in our products ensures that they comply with the regulations for the excepted quantity exemptions of dangerous goods. See the MSDS.
Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4C for a few weeks and still remain viable.
All our goat antibodies are polyclonal and affinity-purified with the immunizing peptide. They are all IgG, and require Protein G and not Protein A, for purification.
Domesticated Nepalese goat, Capra hircus.
US origin, Bovine.
Please refer to “Blocking with the immunizing peptide” protocol, listed on Tech Support page.
Yes, the current batch on sale is on the website. We are continuously working to add additional species and applications to our products, and this information is added to our datasheets when QC is confirmed.
Tested species – species verified as positive by QC testing, citations, or customer feedback, as listed on our datasheets.
Expected species – immunogen peptide sequence shows 100% homology with species, according to NCBI Protein BLAST.
All verified applications are shown on our datasheets – we cannot guarantee it will work in those not listed.
The Quality Control data as presented on the product datasheets are guaranteed. However, different tissue types or cell lines may result in different patterns. For any issues, please refer to relevant protocol listed on Tech Support page, or contact: [email protected].
Please refer to “Western blot Trouble shooting guide” and “Western blot Guidelines” listed on the Tech Support page. Further discussion can be found by following link: non-specific background.
We recommend our affinity purified IgG secondary antibody, which has minimal cross reactivity to human and rodent serum proteins. To purchase please follow the link: /category/secondary-antibodies/
Please refer to the Products tab on Home page.
No. Under our current agreement with Lifespan, the watermark must remain, for these are products which are manufactured by Everest Biotech, but are IHC tested by them. We do not take responsibility for removal of the watermark.
This means that a Cysteine has been added to the peptide sequence, with the sequence then either beginning or ending with C.
Yes. Related peptides can be found on each antibody datasheet.
Yes. The minimum quantity required per order is 1 mg. Please email our Sales team for a quotation: [email protected].
No. We only sell purified antibody.
We do not provide free antibodies. But all of our antibodies are covered by our 100% guarantee. If you are interested in purchasing a smaller sample, please contact: [email protected].
Yes. For pricing please click here.
To check for a distributor in your country, please refer to Distributor tab on Home page. If you can't find your country on the list, please email: [email protected].
No. Unfortunately, we no longer provide this service.
1. Size: Shi-Fix coverslips are 12mm in diameter, and the Shi-Fix Plus are 15mm.
2. Quality: The plus line is made with high quality German glass for high resolution imaging.
3. Culture maintenance: The plus line is suitable for culturing the suspension cells for a day or two prior to staining.
The shelf life for both Shi-fix and Shi-fix plus is one year.
Shi-Fix coverslips (pack sizes of 25 + 50): ø 12mm x 0.17mm (#1.5); Shi-Fix Plus coverslips: ø 15mm x 0.17mm (#1.5). Therefore, the thickness is 0.17mm.
Yes, it is possible to fix micro-organisms, such as E.coli, free mitochondria and organelle membranes. However, mouse embryos have yet to be tested.
Yes, it is possible to measure the metabolic activity of the fixed/ attached cells, as long as the cells are “living”.
We are unable to disclose the composition of the coating material, but it is a combination of mammalian protein and a synthetic organic compound.
We haven’t yet compared Shi-Fix to CELL-TAK, so there is no data. However, our product has been successfully used in many publications and is now endorsed as a standard operating protocol in a Cancer institute of the National institutes of health, USA.
The list of cells that have successfully attached onto the Shifix are as follows: Jurkat cells, K562 cells, U937 cells, Daudi cells, PC12 cells, Raw264.7 cells, MOLT4 cells, PBL culture and Splenocytes.
PBL and K562 have relatively weak attachment on Shi-Fix coverslips. We have Shi-Fix plus coverslips for better attachment of these kind of difficult to attach cell lines.