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Goat Anti-ABCC4 / MRP4 Antibody (EB06538)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06538 | Goat Anti-ABCC4 / MRP4 Antibody | Pep-ELISA, WB, IHC, ICC, IF |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $315.00 |  |
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: multidrug resistance-associated protein 4; multispecific organic anion transporter B, canalicular multispecific organic anion transporter (ABC superfamily), bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4), ATP-binding cassette, sub-family C, member 4, RP11-74A12.1, Multidrug Resistance Protein 4, canalicular multispecific organic anion transporter (ABC superfamily), ATP-binding cassette, sub-family C (CFTR/MRP), member 4, EST170205, MOAT-B, MOATB, MRP4, ABCC4Official Symbol: ABCC4
Accession Number(s): NP_005836.2
Human GeneID(s): 10257
Non-Human GeneID(s): 170924 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Immunogen
Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data obtained from a previous batch and kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4µg/ml.
ICC/IF: This antibody (from a previous batch) has been successfully used in ICC/IF for human primary hepatocytes as described in the following paper: Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G126-35, PMID: 20395535.
Species Reactivity
Tested: Human, RatExpected from sequence similarity: Human, Mouse, Rat
Specific Reference
This antibody has been successfully used in the following papers:
Xu S, Weerachayaphorn J, Cai SY, Soroka CJ, Boyer JL.
Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression.
Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G126-35.
PMID: 20395535