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Goat Anti-ABHD4 (aa 177 to 187) Antibody (EB08070)

Related Products: EBP08070 - Immunizing Peptide, EBP08109 - Immunizing Peptide, Goat Anti-ABHD4 (aa 304 to 316) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08070 Goat Anti-ABHD4 (aa 177 to 187) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08070 1
EB08070 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABHD4, abhydrolase domain containing 4, FLJ12816
Official Symbol: ABHD4
Accession Number(s): NP_071343.2
Human GeneID(s): 63874
Non-Human GeneID(s): 105501 (mouse)

Immunogen

Peptide with sequence C-RPTNPSEIRAP, from the internal region of the protein sequence according to NP_071343.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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