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Goat Anti-ACHE Antibody (EB06659)

Code Name Applications Availability Size Tested species Price Grade  
EB06659 Goat Anti-ACHE Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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ARP American Research Products, Inc
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EB06659 1
EB06659 (0.3µg/ml) staining of Daudi (A), HeLa (B) and Jurkat (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06659 2
EB06659 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06659 3
EB06659 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and nuclear/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB06659 4
EB06659 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase; apoptosis-related acetylcholinesterase
Official Symbol: ACHE
Accession Number(s): NP_000656.1; NP_001269378.1
Human GeneID(s): 43
Important Comments: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).

Immunogen

Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1; NP_001269378.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 65-70kDa band observed in lysates of cell lines Daudi, HeLa and Jurkat, and approx. 70kDa in Rat Brain lysates (calculated MW of 67.8kDa according to Human NP_000656.1 and 68.2kDa according to Rat NP_742006.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Mouse

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