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Goat Anti-ACSL5 Antibody (EB07250)

Related Products: EBP07250 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07250 Goat Anti-ACSL5 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human $300.00 Elite

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Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
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Website: www.axxora.com
EB07250 1
EB07250 (1µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: long-chain fatty acid coenzyme A ligase 5, long-chain acyl-CoA synthetase 5, fatty-acid-Coenzyme A ligase, long-chain 5, FACL5, ACS5, ACS2, HGNC:16526, acyl-CoA synthetase long-chain family member 5, ACSL5
Official Symbol: ACSL5
Accession Number(s): NP_057318.2; NP_976313.1; NP_976314.1
Human GeneID(s): 51703
Important Comments: This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).

Immunogen

Peptide with sequence C-RTQIDSLYEHIQD, from the C Terminus of the protein sequence according to NP_057318.2; NP_976313.1; NP_976314.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in human spleen lysates (calculated MW of 76.0kDa according to NP_976313.1and NP_976314.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N.
Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.
Int J Colorectal Dis. 2005 Apr 5; [Epub ahead of print]
PMID: 15809837

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