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Goat Anti-ADAM17 / TACE Antibody (EB05382)

Related Products: EBP05382 - Immunizing Peptide, Goat Anti-ADAM17 / TACE Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB05382 Goat Anti-ADAM17 / TACE Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB05382 1
EB05382 staining (0.2µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: tumor necrosis factor, alpha, converting enzyme, snake venom-like protease, ADAM metallopeptidase domain 18, CD156B, ADAM18, ADAM metallopeptidase domain 17, snake venom-like protease, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), a disintegrin and metalloproteinase domain 17, TNF-alpha converting enzyme, cSVPADAM metallopeptidase domain 17, TACE, MGC71942, CD156b, CD156b, cSVP, CSVP, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), TACE, ADAM17
Official Symbol: ADAM17
Accession Number(s): NP_003174.3
Human GeneID(s): 6868
Non-Human GeneID(s): 11491 (mouse)
Important Comments: This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).

Immunogen

Peptide with sequence LQRQNRVDSKETEC, from the C Terminus of the protein sequence according to NP_003174.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in human testis lysates (calculated MW of 93.0kDa according to NP_003174.3). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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