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Goat Anti-ADRB1 Antibody (EB07133)

Code Name Applications Availability Size Tested species Price Grade  
EB07133 Goat Anti-ADRB1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB07133 1
EB07133 (0.5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADRB1, adrenergic, beta-1-, receptor, ADRB1R, B1AR, BETA1AR, RHR, beta-1-adrenergic receptor
Official Symbol: ADRB1
Accession Number(s): NP_000675.1
Human GeneID(s): 153

Immunogen

Peptide with sequence ESDEARRCYNDPK, from the internal region of the protein sequence according to NP_000675.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Human Heart lysates (calculated MW of 51.2kDa according to NP_000675.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in the following paper:
Wenzel K, Schulze-Rothe S, Haberland A, Müller J, Wallukat G, Davideit H
Performance and in-house validation of a bioassay for the determination of beta1-auto antibodies found in patients with cardiomyopathy.
Heliyon. 2017 Jul 31;3(7): e00362.
PMID: 28795160

This antibody has been successfully used in Assay Standardization (beating frequency) of Rat Cardiomyocytes:
Wallukat G, Muñoz Saravia SG, Haberland A, Bartel S, Araujo R, Valda G, Duchen D, Diaz Ramirez I, Borges AC, Schimke I.
Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients.
J Am Coll Cardiol. 2010 Feb 2;55(5):463-8.
PMID: 20117461

This antibody has been successfully used in IP on DNA aptamers in vitro:
Haberland A, Wallukat G, Dahmen C, Kage A, Schimke I.
Aptamer neutralization of beta1-adrenoceptor autoantibodies isolated from patients with cardiomyopathies.
Circ Res. 2011 Oct 14;109(9):986-92.
PMID: 21868696

This antibody has been successfully used for In Vitro Testing of Apheresis Column Functionality in Rat:
Wallukat G, Haberland A, Berg S, Schulz A, Freyse EJ, Dahmen C, Kage A, Dandel M, Vetter R, Salzsieder E, Kreutz R, Schimke I.
The first aptamer-apheresis column specifically for clearing blood of β1-receptor autoantibodies.
Circ J. 2012;76(10):2449-55.
PMID: 22850243

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