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Goat Anti-AHI1 / Jouberin Antibody (EB06539)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06539 | Goat Anti-AHI1 / Jouberin Antibody | Pep-ELISA |  |
100µg specific antibody in 200µl | Human | $210.00 |  |
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Target Protein
Principal Names: contatins SH3 and WD40 domains, OTTHUMP00000017265, OTTHUMP00000017264, OTTHUMP00000017263, Ahi-1 isoform I, jouberin, Abelson helper integration site, dJ71N10.1, ORF1, JBTS3, FLJ20069, FLJ14023, DKFZp686J1653, AHI-1, AHI1Official Symbol: AHI1
Accession Number(s): NP_060121.3; NP_001128303.1; NP_001128302.1; NP_001128304.1
Human GeneID(s): 54806
Important Comments: This antibody is expected to recognise both reported isoforms. Variants ( NP_060121.3; NP_001128303.1; NP_001128302.1) encode the same isofom.
Immunogen
Peptide with sequence PTAESEAKVKTC, from the N Terminus of the protein sequence according to NP_060121.3; NP_001128303.1; NP_001128302.1; NP_001128304.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Preliminary experiments gave an approx 22kDa band in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to NP_060121.3. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested:Expected from sequence similarity: Human
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Kennah E, Ringrose A, Zhou LL, Esmailzadeh S, Qian H, Su MW, Zhou Y, Jiang X.
Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells.
Blood. 2009 May 7;113(19):4646-55.
PMID: 19211505
The goat polyclonal antibody used in this paper was manufactured by us:
Zhou LL, Zhao Y, Ringrose A, DeGeer D, Kennah E, Lin AE, Sheng G, Li XJ, Turhan A, Jiang X.
AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells.
J Exp Med. 2008 Oct 27;205(11):2657-71.
PMID: 18936234
The goat polyclonal antibody used in this paper was manufactured by us:
Ringrose A, Zhou Y, Pang E, Zhou L, Lin AE, Sheng G, Li XJ, Weng A, Su MW, Pittelkow MR, Jiang X.
Evidence for an oncogenic role of AHI-1 in Sezary syndrome, a leukemic variant of human cutaneous T-cell lymphomas.
Leukemia. 2006 Sep;20(9):1593-601. Epub 2006 Jul 13.
PMID: 16838023