Change country
Code Name Applications Availability Size Tested species Price Grade  
EB05419 Goat Anti-AIF1 / IBA1 (isoform 1 and 3) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05419 1
EB05419 (1µg/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05419 2
EB05419 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining. This data is from a previous batch, not on sale.
EB05419 3
EB05419 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB05419 4
EB05419 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB05419 5
EB05419 (2µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse), 29427 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 18kDa band observed in Human Brain (Frontal Cortex), 17kDa in Mouse Brain and 16kDa in Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3, and 16.8kDa according to Mouse NP_062340.1 and Rat NP_058892.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Brain for this particular batch.

This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig

Product Reviews

Please login to review this product.