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Code Name Applications Availability Size Species reactivity Price Grade  
EB07174 Goat Anti-AIF1 / IBA1 (isoform 3) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB07174 1
EB07174 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07174 2
EB07174 (1µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ionized calcium-binding adapter molecule, interferon gamma responsive transcript, Em:AF129756.17, IRT-1, AIF-1, DASS-82G15.2, allograft inflammatory factor 1, IBA1, AIF1
Official Symbol: AIF1
Accession Number(s): NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse)
Important Comments: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Immunogen

Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 17kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 1-3µg/ml. Additional bands of unknown identity were also consistently observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Placenta shows strong staining of extracellualr material (with both EB07174 and EB07257). Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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