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Goat Anti-AKAP3 / SOB1 Antibody (EB06311)

Related Products: EBP06311 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06311 Goat Anti-AKAP3 / SOB1 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human $300.00 Elite

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

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San Diego
CA 92121-4727
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Toll Free Tel: 1-800-550-3033
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EB06311 1
EB06311 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: protein kinase A anchoring protein 3, ibrous sheath protein, 95kDa, cancer/testis antigen 82, Fibrous Sheath Protein of 95 kDa, A-kinase anchor protein, 110kDa, A-kinase anchor protein 3, CT82, sperm oocyte binding protein 1, fibrousheathin 1, A kinase (PRKA) anchor protein 3, AKAP110, PRKA3, FSP95, SOB1, AKAP3
Official Symbol: AKAP3
Accession Number(s): NP_006413.2
Human GeneID(s): 10566

Immunogen

Peptide with sequence C-TPLQLLDWLMVNL, from the C Terminus of the protein sequence according to NP_006413.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 95kDa according to NP_006413). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Lefevre A, Duquenne C, Rousseau-Merck MF, Rogier E, Finaz C.
Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition.
Biochem Biophys Res Commun. 1999 May 27;259(1):60-6.
PMID: 10334916

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