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Goat Anti-AKAP9 / AKAP450 / CG-NAP Antibody (EB06654)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06654 | Goat Anti-AKAP9 / AKAP450 / CG-NAP Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse | $315.00 |  |
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$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: AKAP9-BRAF fusion protein, A-kinase anchor protein 9, MU-RMS-40.16A, AKAP450, centrosome- and golgi-localized protein, protein kinase A anchoring protein 9, A-kinase anchor protein, 350kDa, A-kinase anchoring protein 450, kinase N-associated protein, AKAP120-like protein, A kinase (PRKA) anchor protein (yotiao) 9, KIAA0803, HYPERION, AKAP350, YOTIAO, PRKA9, CG-NAP, AKAP9Official Symbol: AKAP9
Accession Number(s): NP_005742.4; NP_671714.1
Human GeneID(s): 10142
Important Comments: This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Immunogen
Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4; NP_671714.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 450kDa band observed in lysates of cell line HepG2 (calculated MW of 454kDa according to NP_ 671700.1). Recommended concentration: 1-3µg/ml.
IHC: The staining pattern observed was identical to that obtained with a rabbit polyclonal antibody specific to AKAP350A (to be submitted to a scientific journal).
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse